Why is it necessary to calibrate the ocular micrometer?

The ocular micrometer should be calibrated for each objective lens and recorded. This is necessary only once for each magnification and each microscope. After such calibrations, microbial size can be measured directly from the ocular micrometer.Click to see full answer. Just so, why is it important to calibrate your microscope?a. Calibrating the microscope is important in being able to measure the objects that are being observed. Using just the lens does not give any reference to the actual size of the object, so using a micrometer on a slide allows for units to be placed and accurate measurements to be taken for the object.Similarly, how do you calibrate an ocular micrometer? To achieve the calibrated measurement of the sample, multiply the measured value of the sample by the labeled magnification of the objective lens divided by the actual magnification ratio. An ocular micrometer is a glass disk with ruled scale on the surface which is etched in chromium. Also Know, why did you have to calibrate the ocular ruler at different total magnifications? The magnification is different for each objective lens. The numerical aperture value holds only for the specific objective ocular lens combination. Ocular space changes with each objective change.What is the purpose of calibrating an ocular micrometer with a stage micrometer?The stage micrometer is used to calibrate an eyepiece reticle when making measurements with a microscope. Eyepiece Reticle (or reticule) -a small piece of glass with a ruler etched into it that fits into a microscope eyepiece. This ruler is used to make measurements of objects viewed through the microscope.

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